博士，研究员

联系邮箱：huangjc65@szu.edu.cn

教育背景：

2001/6 – 2003/5：香港大学，藻类生物技术，博士后

1997/6 - 2000/11：香港大学，分子遗传学，博士研究生

1989/9 - 1992/6：中山大学，遗传工程，硕士研究生

1983/9 - 1987/6：华南师范大学，生物学，本科生

工作经历：

2020/7 - 现在：深圳大学高等研究院，研究员

2011/2 - 2020/6：中国科学院昆明植物研究所，资源植物与生物技术实验室，研究员，博士生导师，课题组组长

2003/6 - 2011/1：香港大学生物科学院，植物系，高级助理研究员

1992/7 - 1997/5：中山大学，生物工程研究中心，助教、讲师

1987/7 - 1989/9：惠州学院，生物系，助教

学术兼职：

云南省细胞生物学学会理事.

云南省植物学会理事

Algal Research, Journal of Agricultural and Food Chemistry, Journal of Phycology, Planta等审稿人

所获奖项：

2013年云南省高端人才计划

2013年云南省引进海外高层次人才计划

研究兴趣：

1、单细胞绿藻虾青素代谢调控机制的研究

阐明雨生红球藻（Haematococcus pluvialis）和小球藻（Chlorella zofingiensis）差异合成和积累虾青素的机制，为通过基因工程集合优化它们的优良性状以大大提高其虾青素生产效率提供理论指导。

2、以藻类和植物为细胞工厂生产虾青素的代谢工程研究

藻类和植物的叶绿体和有色体含有大量的非酮化类胡萝卜素，可进一步被催化成虾青素。然而植物高效表达典型β-胡萝卜素酮化酶（BKT）基因未能积累高量虾青素。主要原因是植物内源高活性的β-胡萝卜素羟化酶（BHY）使羟化β-胡萝卜素占绝对优势而典型的BKT难以催化羟化β-胡萝卜素成虾青素。分离和功能分析新型BKT是解决这一难题的关键所在。本实验室已从不同藻中分离到多个BKT 基因并得到功能增强的新型BKT基因，为实现在重要藻类和经济作物积累具有商业化产量虾青素提供可能。

3、微藻脂肪酸和油脂合成的调控研究

单细胞藻类广泛生长于自然界中不同的生态环境中，它们是最大的初级生产物质生物类群。较之植物，藻类具有生长快，含油量高的优点。然而野生型藻类常常表现出生长快但含油量低或含油量高却生长慢。因此，选育出生长快含油量高的藻种是开发生物能源的关键。本研究组从不同的生境中筛选生长速度快含油量高的藻种，如小球藻属 (Chlorella) 中的特异种，对其生长特性，脂肪酸和其他代谢进行研究。在此基础上，利用基因工程或遗传诱变优化其生长及脂肪酸的合成，以期得到高抗逆, 高产油，可规模化生产生物柴油的工程藻种。

科研项目：

（1）云南省高端科技人才引进项目（2012HA017）：植物产业化生产虾青素的代谢工程，2012年12月至2016年11月，200万，主持人；

（2）云南省科技厅重点项目（39Y435531261）：高虾青素含量番茄新品种选育，2014年11月-2017年10月，60万，主持人；

（3）云南省基础应用重点项目（31Y43E531261）：食用小球藻合成和积累虾青素的代谢工程，2014年12月-2017年11月，50万，主持人。

（4）山东省中昆生物技术有限公司横向课题：高产虾青素番茄产业化研究，2014-2017，100万，主持人。

（6）中国科学院“百人计划”:虾青素生物合成， 2011年7月至2014年6月，200万，主持人。

（6）中国科学院昆明植物研究所引进海外杰出人才项目：虾青素代谢工程，2011年2月至2014年1月，100万，主持人。

发表论文：

1. Li Y.Y. and Huang J.C.* (2020) Charaterization of an algal phosphomannose isomerase gene and its application as a selectable marker for genetic manipulation of tomato. https://doi.org/10.1016/j.pld.2020.06.001

2. Gong Y. and Huang J.C.* (2020) Characterization of four untapped microalgae for the production of lipids and carotenoids. Algal Research 49:101897.

3. Ye Y. and Huang J.C.＊(2019) Defining the biosynthesis of ketocarotenoids in Chromochloris zofingiensis. Plant Diversity 1:61-66.

4. Lujun Yu, Qinfang Chen, Yujun Peng, Lijuan Xie, Di Liu, Muqian Han, Feng Chen, Shi Xiao,Junchao Huang　J.C.* and Juan Li*（２０１９）Arabidopsis thaliana Plants Engineered To Produce Astaxanthin Show Enhanced Oxidative Stress Tolerance and Bacterial Pathogen Resistance．Journal of Agriculture and Food Chemistry　67:12590-12598.

5. Liu M.M., Sandmann G., Chen F. and Huang J.C.* (2019) Enhanced coproduction of cell-bound 　zeaxanthin and secreted exopolysaccharides by Sphingobium sp. via metabolic engineering and optimized fermentation. Journal of Agriculture and Food Chemistry67:12228-12236.

6. Ye J. R., Liu M. M., He M. X., Ye Y. and Huang J. C.* (2019) Defining and enhancing the biosynthesis of astaxanthin and docosahexaenoic acid in Aurantiochytrium sp. SK4. Marine Drugs 17(45):1-13.

7. Huang W. P., Lin Y., He M. X., Gong Y.H., and Huang J.C* (2018) Induced high-yield production of zeaxanthin, lutein, and β-carotene by a mutant of Chlorella zofingiensis. Journal of Agriculture and Food Chemistry 66:891-897.

8. Li S. and Huang J.C.* (2018) Assessment of expression cassettes and culture media for different Escherichia coli strains to produce astaxanthin. Natural Products and Bioprospecting, 8:397-403.V)

9. Huang W. P., Ye J.R., Lin Y., Zhang J.J., He M. and Huang J.C* (2016) Transcriptome analysis of Chlorella zofingiensis to identify genes and their expressions involved in astaxanthin and triacylglycerol biosynthesis. Algal Research 17:236-243.

10. Suen Y.L., Tang H.M., Huang J.C*.， and Chen F*. (2014) Enhanced production of fatty acids and astaxanthin in Aurantiochytrium sp. by the expression of Vitreoscilla hemoglobin. Journal of Agriculture and Food Chemistry62:12392-12398.

11. Huang J.C, Zhong Y.J, Liu J., Sandmann G, Liu J and Chen F* (2013) Metabolic engineering of tomato for high-yield production of astaxanthin. Metabolic Engineering 17:59-67.

12. Huang J.C, Zhong Y.J, Sandmann G, Liu J and Chen F* (2012) Cloning and selection of carotenoid ketolase genes for the engineering of high-yield astaxanthin in plants. Planta 236:691-699.

13. Liu J, Huang J.C*, Jiang Y and Chen F* (2012) Molasses-based growth and production of oil and astaxanthin by Chlorella zofingiensis. Bioresource Technology107:393-398.

14. Zhong Y-J, Huang J.C *, Liu J, Li Y, Jiang Y, Xu Z-F, Sandmann G, and Chen F* (2011) Functional characterization of various algal carotenoid ketolases reveals that ketolating zeaxanthin efficiently is essential for high production of astaxanthin in transgenic Arabidopsis. Journal of Experimental Botany 62：3659-69.

15. Liu J, Huang JC and Chen F (2011). Microalgae as Feedstocks for Biodiesel Production, Biodiesel - Feedstocks and Processing Technologies, Margarita Stoytcheva and Gisela Montero (Ed.), ISBN: 978-953-307-713-0, InTech.

16. Liu J, Huang JC*, Sun Z, Zhong Y, Jiang Y,and Chen F* (2011) Differential lipid and fatty acid profiles of photoautotrophic and heterotrophic Chlorella zofingiensis: Assessment of algal oils for biodiesel production. Bioresource Technology. 102:106-110.

17. Liu J, Huang JC*, Fan K.W, Jiang Y, Zhong Y, Sun Z, and Chen F* (2010) Production potential of Chlorella zofingiensis as a feedstock for biodiesel. Bioresource Technology. 101:8658-63.

18. Liu J, Zhong Y, Sun Z, Huang JC*, Sandmann G, and Chen F* (2010) One amino acid substitution in phytoene desaurase makes Chlorella zofingiensis resistant to norflurazon and enhances the biosynthesis of astaxanthin. Planta 232:61-67.

19. Li AM, Yu bY, Chen FH, Gan HY, Yuan JG Qiu RL, Huang JC, Yang ZY and Xu ZF (2009) Characterization of the Sesbania rostrata phytochelatin synthase gene: Alternative splicing and function of four isoforms. International Journal of Molecular Sciences 10:3269-3282.

20. Li YT, Huang JC*, Sandmann G and Chen F* (2009) High-light and sodium chloride stress differently regulate the biosynthesis of astaxanthin in Chlorella zofingiensis (Chlorophyceae). Journal of Phycology 45:635-641.

21. Li YT, Huang JC*, Sandmann G and Chen F* (2008) Glucose sensing and the mitochondrial alternative pathway are involved in the regulation of astaxanthin biosynthesis in the dark-grown Chlorella zofingiensis (Chlorophyceae). Planta 228:735-743.

22. Sun N, Wang Y, Li YT, Huang JC* and Chen F* (2008). Sugar-based growth, astaxanthin accumulation and carotenogenic transcription of heterotrophic Chlorella zofingiensis (Chlorophyta). Process Biochemistry, 43, 1288-1292.

23.Huang JC, Liu J, Li YT and Chen F* (2008). Isolation and characterization of the phytoene desaturase gene as a potential selective marker for genetic engineering of the astaxanthin-producing green alga Chlorella zofingiensis (Chlorophyta). Journal of Phycology, 44, 684-690.

24.Huang JC, Wang Y, Sandmann G* and Chen F* (2006) Isolation and characterization of a carotenoid oxygenase gene from Chlorella zofingiensis (Chlorophyta). Applied Microbiology and Biotechnology 71:473-479.

25.Huang JC, Sandmann G and Chen F* (2006) Stress-related differential expression of multiple b-carotene ketolase genes in the unicellular green alga Haematococcus pluvialis. Journal of Biotechnology 122:176-185.

26.Huang JC and Chen F*(2006) Simultaneous amplification of 5′ and 3′ cDNA ends based on template-switching effect and inverse PCR. Biotechniques 40:187-189.

27. Lai JP, Jiang Y, He XW, Huang JC and Chen F* (2004). Separation and determination of astaxanthin from microalgal and yeast samples by molecularly imprinted microspheres. Journal of Chromatography B, 804: 25-30.

28. HuangJC, Corke H and Sun M* (2002) Phylogenetic relationships of sweetpotato and its wild relatives in Ipomoea series Batatas (Convolvulaceae) detected by AFLPs and ITS sequence. Genetic Resources and Crop Evolution 49: 541-550.

29. Chen G, Ye CM, Huang JC, Yu M and Li BJ* (2001) Cloning of the papaya ringspot virus (PRSV) replicase (RP) gene and generation of PRSV-resistant papayas through introduction of PRSV replicase gene. Plant Cell Reports, 20: 272-277.

30. HuangJC and Sun M* (2000) Fluorecene PAGE analysis of Microsatellite-primed PCR: a fast and efficient approach for genomic fingerprinting. Biotechniques 28: 1068-1072.

31.Huang JC and Sun M* (2000) Genetic diversity and relationships of sweetpotato and its wild relatives in Ipomoea series Batatas (Convolvulaceae) as revealed by inter-simple sequence repeat (ISSR) and restriction analysis of chloroplast DNA, Theoretical and Applied Genetics 100: 1050-1060.

32.Huang JC, Ge XJ and Sun M* (2000) A modified CTAB protocol using a silica matrix for isolation of plant genomic DNA. Biotechniques 28: 432-434.

33. Li WD, Huang JC and Corke H* (2000) Effect of b–cyclodextrin on pasting properties of wheat starch. Nahrung –Food 44: 164-167. (changed to Molecular Nutrition and Food Research)

授权专利：

1 一种产玉米黄素和β-胡萝卜素小球藻突变株及其培养方法（ZL 2016110887200.0）

2 转基因番茄果实中虾青素的超临界二氧化碳流体萃取方法（ZL 201710452369.X）

3 利用转基因植物生产虾青素的方法（ZL 20120223297.9）

4 转基因番茄的组织培养方法(ZL 201310300186.8)

5 一种提高含虾青素转基因番茄产量和转基因番茄中虾青素含量的方法（ZL 201410403342.8）

6 一种促进转基因番茄生长和虾青素积累的栽培方法（ZL 2014104035601）

7 一种叶产虾青素番茄品种的培育方法（ZL 2016 1 0003043.4）

8 叶产虾青素番茄品种的培养方法（ZL 2016 1 0003289.1）

9 叶产虾青素番茄的培育方法（ZL 2016 1 0003570.5）

受理专利：

10- 一种鞘氨醇单胞菌遗传转化的方法（申请号201610657389.6）

11- 鞘氨醇单胞菌及其生产类胡萝卜素的方法（201610656211.X）

学术报告及会议：

2014.06, 第二届中德代谢组研讨会，德国柏林，邀请报告：The biosynthesis of astaxanthin in Chlorella zofingiensis.